Immunolocalisation of nitric oxide synthase isoforms in the ductuli efferentes and epididym
نویسندگان
چکیده
منابع مشابه
Structure and function of the ductuli efferentes: a review.
INTRODUCTION The ductuli efferentes are a series of tubules that conduct sperm from the rete testis to the epididymis . These small ducts are unique because they are the only region of the male reproductive tract that is lined by a ciliated epithelium. The presence of cilia seemed to have provided the impetus for their study starting with Becker in 1856 and 1857, who first described the cylindr...
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Nitric oxide (NO) production in the rat placenta was monitored and quantified by electron paramagnetic resonance (EPR) spectroscopy with hemoglobin and an Fe-N-(dithiocarboxy)sarcosine (DTCS) complex as NO-trapping reagents. Expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative RT-PCR analysis. The EPR spectrum of the placenta with hemoglobin trapping showed a thr...
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Experiments were performed to quantify nitric oxide synthase (NOS) activity and identify the NOS isoforms present in the Sprague-Dawley rat renal vasculature. NOS enzymatic activity was measured by adding [(3)H]arginine to microdissected renal blood vessels and quantifying the conversion to [(3)H]citrulline by reverse-phase high-performance liquid chromatography. Total NOS activity was greatest...
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The present research used immunohistochemistry to analyse the detection and localization of NOS isoforms in the ductuli efferentes and epididymis of prepubertal and adult alpaca. In the ductuli efferentes and epididymis of prepubertal and adult animals, nNOS and eNOS were similarly expressed in epithelial lining cells, conversely differences were observed in the immunopresence of iNOS. Our data...
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Nitric oxide (NO) produces rapid osteoclast detachment and contraction in vitro, and this effect is accompanied by a profound inhibition of bone resorption. Work by others has confirmed these findings in vivo: inhibition of NO synthase [NOS; L-arginine, NADPH: oxygen oxidoreductase (NO-forming), EC 1.14.13.39] in normal rats is followed by increased bone resorption reflected by a marked loss in...
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ژورنال
عنوان ژورنال: Folia Morphologica
سال: 2017
ISSN: 1644-3284,0015-5659
DOI: 10.5603/fm.a2017.0046